Produções dos Docentes

Professor’s Publications

  • SAFETY AND IMMUNOGENICITY OF THE TETRAVALENT, LIVE-ATTENUATED DENGUE VACCINE BUTANTAN-DV IN ADULTS IN BRAZIL: A TWO-STEP, DOUBLE-BLIND, RANDOMISED PLACEBO-CONTROLLED PHASE 2 TRIAL, Lancet Infectious Disease (impact factor 24.446).

This paper evaluated the safety and immunogenicity of the tetravalent vaccine for Dengue produced at Instituto Butantan The authors showed that the vaccine is safe and capable of inducing a robust production of antibodies against dengue virus serotypes.

 

  • IMMUNOPROTECTIVE ACTIVITY INDUCED BY LEPTOSPIRAL OUTER MEMBRANE PROTEINS IN HAMSTER MODEL OF ACUTE LEPTOSPIROSIS, Frontiers in Immunology (impact factor 6.429).

In this paper, the group investigated the immunoprotective effect of eight recombinant proteins from the Leptospira bacterium. Of these, two (rLIC3259 and rLIC11711) proved to be protective. The authors showed that these proteins are involved with the protective capacity via factors of the human complement system.

 

  • STRATEGIES USED BY SPIROCHETES TO EVADE THE HOST COMPLEMENT SYSTEM, FEBS Letter (impact factor: 3.057).

This is a review paper in which the authors discuss the role of the complement system in the eradication of the Leptospira bacterium and the strategies adopted by Leptospira to circumvent this complement system for its own benefit.

  • In 2020, one of our professors generated all the documentation for the Virology Laboratory of the Instituto Butantan, aiming at the traceability of activities. Furthermore, she managed all the certification and calibration of equipment and instruments. She also prepared all the paperwork (POPs) to obtain the laboratory's biosafety certificate.

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  • EXTRACELLULAR VESICLES ISOLATED FROM MESENCHYMAL STROMAL CELLS MODULATE CD4+T LYMPHOCYTES TOWARD A REGULATORY PROFILE, Cells (impact factor: 4.366).

In the context of induction of adaptive immune response by different microorganisms or maintenance of people's homeostasis. This paper showed the capacity of mesenchymal stromal cells (MSC), via microvesicle release, to modulate the activation and differentiation of T lymphocytes, which are directly involved in adaptive and effector immunity. Furthermore, the results presented suggest the mechanism involved in the modulatory effect of mesenchymal cells in inducing regulatory T cell differentiation.

 

  • GENOMIC AND PHENOTYPIC CHARACTERIZATION OF ANTIMICROBIAL RESISTANCE IN CARBAPENEM-RESISTANT ACINETOBACTER BAUMANNII HYPERENDEMIC CLONES CC1, CC15, CC79, AND CC25, International Journal of antimicrobial agents (impact factor: 4.621).

By sequencing 79 samples of Acinetobacter baumannii (CRAB) recovered from 26 Brazilian hospitals, this study provides information to understand the resistance of these samples to antimicrobials.

 

  • CLINICAL PROFILE OF CONFIRMED SCORPION STINGS IN A REFERRAL CENTER IN MANAUS, WESTERN BRAZILIAN AMAZON, Toxicon (impact factor: 2.201).

This paper described the clinical and epidemiological aspects of 151 confirmed cases of scorpion stings, which were treated in two hospitals in Amazonas, between 2014 and 2019. The paper suggests that the available antivenoms have little effectiveness when used in cases of poisoning by certain scorpions.

 

  • DEVELOPMENT OF RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (NARTOGRASTIM) PRODUCTION PROCESS IN ESCHERICHIA COLI COMPATIBLE WITH INDUSTRIAL SCALE AND WITH NO ANTIBIOTICS IN THE CULTURE MEDIUM, Applied Microbiology and Biotechnology (impact factor: 3.913).

This paper presents a purification process for nartograstim, a variant of the cytokine “granulocyte colony-stimulating factor”, with high purity, structural integrity and biological activity.

 

  • IMPROVEMENTS ON THE QUANTITATIVE ANALYSIS OF TRYPANOSOMA CRUZI HISTONE POST TRANSLATIONAL MODIFICATIONS: STUDY OF CHANGES IN EPIGENETIC MARKS THROUGH THE PARASITE'S METACYCLOGENESIS AND LIFE CYCLE, Journal of Proteomics (impact factor: 3.509).

This paper analyzed post-translational modifications of histones during the differentiation of Trypanosoma cruzi, the etiologic agent of Chagas disease. The group observed that the pattern of modifications changes during this process and, by describing these alterations, they brought up for discussion the role of histone modifications and, therefore, of the chromatin structure, in the life cycle of T. cruzi.



  • IMMUNIZATION WITH PHTD TRUNCATED FRAGMENTS REDUCES NASOPHARYNGEAL COLONIZATION BY STREPTOCOCCUS PNEUMONIAE, Vaccine (impact factor: 3.143).

In this paper, the immune response in mice systemically immunized with amino and carboxy-terminal regions of protein D (PhtD) of Streptococcus pneumoniae was characterized. Immunization protected against pneumococcal colonization, including the N-terminal portion of PhtD as a vaccine candidate.

 

  • REPLICATION ORIGIN LOCATION MIGHT CONTRIBUTE TO GENETIC VARIABILITY IN TRYPANOSOMA CRUZI, BMC genomics (impact factor: 3.594)

This paper identified the location of replication origins in the genome of Trypanosoma cruzi, the etiologic agent of Chagas disease, verifying that the origins are favoring a potential collision between the replication and transcription machinery. Thus, the work raises the possibility that the location of origins is directly involved with the genetic variability found in this organism.

 

  • PREVALENCE OF ENTEROPATHOGENS AND VIRULENCE TRAITS IN BRAZILIAN CHILDREN WITH AND WITHOUT DIARRHEA, Frontiers in cellular and infection microbiology (impact factor: 4.300).

 

This paper characterized the presence of diarrhea-causing bacteria, E. coli, Salmonella spp and Yersinia enterocolitica, in samples from children with or without diarrheal episodes using the quantitative real-time PCR (qPCR) methodology. The data showed that asymptomatic children from Brazilian urban areas can become reservoirs for these enteropathogens.



  • METALLOPROTEINASE SUPPRESSION DRIVEN BY THE CURCUMIN ANALOG DM-1 MODULATES INVASION IN BRAF-RESISTANT MELANOMAS, anti-cancer agents in medicine chemistry (impact factor: 2.369).

This paper evaluated the stability and cytotoxic effect of DM-1, a curcumin analogue, in melanomas that are sensitive and resistant to a frequent mutation in melanomas (BRAF V600E). Subtoxic doses of DM-1 were able to decrease the expression of metalloproteinases related to tumor invasion. Thus, this paper opens opportunities for further studies to study the combination of drugs for the treatment of melanomas.

 

  • PHARMACOLOGICAL INHIBITION OF LYSINE-SPECIFIC DEMETHYLASE 1 (LSD1) INDUCES GLOBAL TRANSCRIPTIONAL DEREGULATION AND ULTRASTRUCTURAL ALTERATIONS THAT IMPAIR VIABILITY IN SCHISTOSOMA MANSONI, Plos Neglected Tropical Disease (impact factor: 4.400).

In this study, the group studied the effect of the synthetic inhibitor MC3935 on the lysine-specific enzyme of Schistosoma mansoni demethylase 1 (SmLSD1). The treatment affected the parasite motility, the integument and cell structures, culminating in the death of the worms. Thus, the data showed that the SmLSD1 enzyme is a promising drug target for the treatment of schistosomiasis.

 

  • LOWER COST ALTERNATIVES FOR MOLECULAR DIAGNOSIS OF COVID-19: CONVENTIONAL RT-PCR AND SYBR GREEN-BASED RT-QPCR, Brazilian Journal of Microbiology (impact factor: 2,428).

In this paper, the authors standardized low-cost molecular diagnostic methods for identifying SARS-CoV-2.

 

  • OPTIMIZATION AND SCALE-UP PRODUCTION OF ZIKA VIRUS -NS1 IN ESCHERICHIA COLI: APPLICATION OF RESPONSE SURFACE METHODOLOGY, AMB express (impact factor: 2.499).

This paper optimized the expression of the C-terminal region of the soluble Zika virus NS1 antigen in order to use this antigen in serological tests to differentiate ZIKA infection from other flavivirus infections.

 

  • CORONAVIRUS: ANTI-NEGATIONIST LESSONS AND THE FUTURE OF THE PLANET, Scielo em Perspectiva.

In this paper, the authors describe the coronavirus pandemic, discuss the need for countries to accept the serious situation and trust science, and reflect on the need for global solidarity to build a better future.



  • ANALYSIS OF THE VIRULENCE PROFILE AND PHENOTYPIC FEATURES OF TYPICAL AND ATYPICAL ENTEROAGGREGATIVE ESCHERICHIA COLI (EAEC) ISOLATED FROM DIARRHEAL PATIENTS IN BRAZIL, Frontiers in cellular and infection microbiology (impact factor: 4.300).

This paper presents an extensive characterization of 220 isolates of enteroaggregative E. coli bacteria (EAEC) obtained from patients with diarrhea during the years 2010 and 2016. In this way, it was possible to identify the afp genes as potential markers for the diagnosis of EAEC.

 

  • GENOMIC PROPERTIES AND TEMPORAL ANALYSIS OF THE INTERACTION OF AN INVASIVE ESCHERICHIA ALBERTII WITH EPITHELIAL CELLS, Frontiers in cellular and microbiology infection (impact factor: 4.300).

This paper investigated the contribution of effectors of the type three secretion system (T3SS) in the process of invasion of the 1551-2 strain of the bacterium Escherichia albertii, one of the causes of infant mortality, evaluating the expression of LEE operons at various stages of the interaction of the bacterium with to Caco-2 intestinal cells. The paper draws attention to the importance of T3SS during the process of invasion and maintenance of bacteria in the intracellular space.